abstract：OBJECTIVE:Establishment of a clonal bipotent chondroprogenitor cell line from adult mouse to provide a new tool for the elucidation of chondrogenesis in adult animal. DESIGN:A clonal cell line CL-1 was established from tibia of adult mouse. Differentiation of CL-1 was characterized in monolayer culture. Effects of gro...

journal_title：Osteoarthritis and cartilage

authors： Kitamura H

更新日期：2004-01-01 00:00:00

abstract：:Although the multifactorial nature of osteoarthritis (OA) is well recognized, genetic factors have been found to be strong determinants of the disease. Evidence of a genetic influence of OA comes from a number of sources, including epidemiological studies of family history and family clustering, twin studies, and expl...

journal_title：Osteoarthritis and cartilage

authors： Spector TD,MacGregor AJ

更新日期：2004-01-01 00:00:00

abstract：OBJECTIVE:To characterise longitudinal changes in joint integrity and cartilage volume in vivo in the guinea pig spontaneous osteoarthritis (OA) model by magnetic resonance imaging (MRI). METHODS:Guinea pigs knee were imaged in vivo by high-resolution three-dimensional (3D) MRI between the ages of 3 and 12 months. Ima...

journal_title：Osteoarthritis and cartilage

authors： Tessier JJ,Bowyer J,Brownrigg NJ,Peers IS,Westwood FR,Waterton JC,Maciewicz RA

更新日期：2003-12-01 00:00:00

abstract：OBJECTIVE:To assess if various biological responses to transforming growth factor-beta (TGF-beta) in chondrocytes are differentially regulated by Smad-6 and Smad-7. DESIGN:Adenoviral overexpression of Smad-6 or -7 mRNA in a chondrocyte cell line was determined via semi-quantitative RT-PCR and protein overexpression wa...

journal_title：Osteoarthritis and cartilage

authors： Scharstuhl A,Diepens R,Lensen J,Vitters E,van Beuningen H,van der Kraan P,van den Berg W

更新日期：2003-11-01 00:00:00

abstract：OBJECTIVE:To quantify the precision and accuracy of measurements of joint space width (JSW) and joint space narrowing (JSN) from the medial tibiofemoral compartment of knee radiographs using a simple and easily adaptable protocol. METHODS:Radiographs of a caliper (a surrogate for JSW) were obtained to determine the pr...

journal_title：Osteoarthritis and cartilage

authors： Dupuis DE,Beynnon BD,Richard MJ,Novotny JE,Skelly JM,Cooper SM

更新日期：2003-10-01 00:00:00

abstract：OBJECTIVE:To examine the effect of IL-1 beta-induced *NO and PGE(2)release by stimulated superficial and deep chondrocyte/agarose constructs subjected to mechanical compression. DESIGN:Chondrocyte sub-populations were seeded separately in agarose constructs and cultured unstrained, within a 24-well tissue culture plat...

journal_title：Osteoarthritis and cartilage

authors： Chowdhury TT,Bader DL,Lee DA

更新日期：2003-09-01 00:00:00

abstract：OBJECTIVE:The aim of the study was to assess the inter-observer reliability and intra-observer reproducibility of the Ahlbäck radiographic classification for knee osteoarthritis (OA). DESIGN:Ninety-six knee radiographs of patients with clinical signs of arthritis were evaluated and classified into five grades of Ahlbä...

journal_title：Osteoarthritis and cartilage

authors： Galli M,De Santis V,Tafuro L

更新日期：2003-08-01 00:00:00

abstract：OBJECTIVE:To validate quantitative magnetic resonance imaging (qMRI) for the assessment of cartilage volume and thickness in thin and curved cartilage layers, such as the shoulder. METHODS:Eight shoulder specimens from healthy individuals (aged 31-69 years) were investigated using a 3D gradient echo sequence with sele...

journal_title：Osteoarthritis and cartilage

authors： Graichen H,Jakob J,von Eisenhart-Rothe R,Englmeier KH,Reiser M,Eckstein F

更新日期：2003-07-01 00:00:00

abstract：OBJECTIVE:To characterise in vitro engineered cartilaginous constructs made employing a novel static, scaffold-free and closed chamber system. DESIGN:Chondrocytes derived from slaughter age pigs (3-6 months) were seeded at high density (20 x 10(6)) into cylindrical chambers (1.0 x 0.5cm) and were maintained between an...

journal_title：Osteoarthritis and cartilage

authors： Grogan SP,Rieser F,Winkelmann V,Berardi S,Mainil-Varlet P

更新日期：2003-06-01 00:00:00

abstract：OBJECTIVE:This study was designed to systematically determine whether and to what extent the frequency of intermittent loading modulates the biosynthesis and release of proteoglycans (PGs), and to assess chondrocyte viability within mature bovine articular cartilage explants exposed to different loading patterns. METH...

journal_title：Osteoarthritis and cartilage

authors： Sauerland K,Raiss RX,Steinmeyer J

更新日期：2003-05-01 00:00:00

abstract：OBJECTIVE:Cartilage swelling is an early event in osteoarthritis (OA). However, the response of chondrocytes to increased tissue hydration is unknown. This work studied the volume and morphology of living in situ human chondrocytes as a function of cartilage degeneration. METHODS:The tibial plateaus from knee joints o...

journal_title：Osteoarthritis and cartilage

authors： Bush PG,Hall AC

更新日期：2003-04-01 00:00:00

abstract：OBJECTIVE:The aim of this study was to investigate the effects of hypo-osmotically induced calcium (Ca(2+)) transients on the organization of the actin cytoskeleton in articular chondrocytes. The secondary hypothesis tested was that actin restructuring following hypo-osmotic stress is mediated by gelsolin. METHODS:Iso...

journal_title：Osteoarthritis and cartilage

authors： Erickson GR,Northrup DL,Guilak F

更新日期：2003-03-01 00:00:00

abstract：OBJECTIVE:Osteophytes are non-neoplastic osteo-cartilaginous protrusions growing at the margins of osteoarthritic joints. They can not only be considered as in situ repair tissue, but also represent an excellent in vivo model for induced cartilage repair processes. Our focus was to identify different steps of osteophyt...

journal_title：Osteoarthritis and cartilage

authors： Gelse K,Söder S,Eger W,Diemtar T,Aigner T

更新日期：2003-02-01 00:00:00

abstract：OBJECTIVE:Partial meniscectomy is the golden standard for treating a bucket-handle tear in the meniscus of the knee, but it inevitably leads to articular cartilage degeneration. Surgical creation of an access channel between the lesion and the vascularized synovial lining is intended to induce ingrowth of repair tissue...

journal_title：Osteoarthritis and cartilage

authors： van Tienen TG,Heijkants RG,de Groot JH,Pennings AJ,Poole AR,Veth RP,Buma P

更新日期：2003-01-01 00:00:00

abstract：OBJECTIVES:Osteoarthritis (OA) is the most prevalent chronic disease in the elderly, and it is generally diagnosed at an advanced state when treatment is difficult if not impossible. The early form of OA is characterized by an elevated water content in the cartilage tissue. The purpose of this study was to verify in vi...

journal_title：Osteoarthritis and cartilage

authors： Liess C,Lüsse S,Karger N,Heller M,Glüer CC

更新日期：2002-12-01 00:00:00

abstract：OBJECTIVE:To develop a reliable, valid, and responsive self-administered questionnaire to probe pain, stiffness and physical disability in patients with osteoarthritis (OA) of the hand. DESIGN:In order to assess the dimensionality of the symptomatology of hand OA, a self-administered questionnaire was developed to pro...

journal_title：Osteoarthritis and cartilage

authors： Bellamy N,Campbell J,Haraoui B,Buchbinder R,Hobby K,Roth JH,MacDermid JC

更新日期：2002-11-01 00:00:00

abstract：OBJECTIVE:To elucidate variations in tissue ultrastructure and incidence of pathology between fibromodulin (FM)-null mice and wild-type (WT) animals. DESIGN:FM-null and WT siblings from different age groups were compared. Serial sections were made through paraffin-embedded whole knees and investigated histologically. ...

journal_title：Osteoarthritis and cartilage

authors： Gill MR,Oldberg A,Reinholt FP

更新日期：2002-10-01 00:00:00

abstract：AIM:The goal of this study was to determine whether or not the intraarticular administration of hyaluronic acid can improve functional parameters, such as isokinetic muscle strength or total work and clinical test results in patients with osteoarthritis (OA) of the knee. METHOD:As part of a prospective, controlled stu...

journal_title：Osteoarthritis and cartilage

authors： Miltner O,Schneider U,Siebert CH,Niedhart C,Niethard FU

更新日期：2002-09-01 00:00:00

abstract：OBJECTIVE:We evaluated C-reactive protein (C-RP), a quantitative marker of the acute phase response, as a potential biomarker of prevalent and incident osteoarthritis of the knee (OAK). METHODS:Serum C-reactive protein concentrations were characterized with ultrasensitive rate nephelometry in a population-based sample...

journal_title：Osteoarthritis and cartilage

authors： Sowers M,Jannausch M,Stein E,Jamadar D,Hochberg M,Lachance L

更新日期：2002-08-01 00:00:00

abstract：OBJECTIVE:First, to assess the clinical effectiveness of hylan G-F 20 in an appropriate care treatment regimen (as defined by the American College of Rheumatology (ACR) 1995 guidelines) as measured by validated disease-specific outcomes and health-related quality of life endpoints for patients with osteoarthritis (OA) ...

journal_title：Osteoarthritis and cartilage

authors： Raynauld JP,Torrance GW,Band PA,Goldsmith CH,Tugwell P,Walker V,Schultz M,Bellamy N,Canadian Knee OA Study Group.

更新日期：2002-07-01 00:00:00

abstract：OBJECTIVE:Protective effects of SKI 306X, a natural herbal product extracted from three herbs Clematis mandshurica, Trichosanthes kirilowii, and Prunella vulgaris, on articular cartilage was examined and compared with other osteoarthritis (OA) drugs using in vitro and in vivo models. METHODS:In vitro culture of rabbit...

journal_title：Osteoarthritis and cartilage

authors： Choi JH,Choi JH,Kim DY,Yoon JH,Youn HY,Yi JB,Rhee HI,Ryu KH,Jung K,Han CK,Kwak WJ,Cho YB

更新日期：2002-06-01 00:00:00

abstract：OBJECTIVE:We investigated whether chondrocytes derived from osteoarthritic cartilage may lose their responsiveness to cartilage-derived morphogenetic protein-1, -2 (CDMP-1, -2) and osteogenic protein-1 (OP-1) compared with healthy cells, thus leading to an impaired maintenance of matrix integrity. DESIGN:Chondrocytes ...

journal_title：Osteoarthritis and cartilage

authors： Bobacz K,Gruber R,Soleiman A,Graninger WB,Luyten FP,Erlacher L

更新日期：2002-05-01 00:00:00

abstract：OBJECTIVE:It has been suggested that osteoarthritis (OA) is induced by mechanical stress followed by cartilage destruction, and it is generally accepted that there is little involvement of an immune response in OA compared with that in rheumatoid arthritis (RA). We have previously found clonally expanded transcripts of...

journal_title：Osteoarthritis and cartilage

authors： Ishii H,Tanaka H,Katoh K,Nakamura H,Nagashima M,Yoshino S

更新日期：2002-04-01 00:00:00

abstract：OBJECTIVE:There is no widely accepted method to repair articular cartilage defects. Bone marrow mesenchymal cells have the potential to differentiate into bone, cartilage, fat and muscle. Bone marrow mesenchymal cell transplantation is easy to use clinically because cells can be easily obtained and can be multiplied wi...

journal_title：Osteoarthritis and cartilage

authors： Wakitani S,Imoto K,Yamamoto T,Saito M,Murata N,Yoneda M

更新日期：2002-03-01 00:00:00

abstract：OBJECTIVE:One of the main open questions in chondrocyte transplantation is the fate of the implanted cells in vivo. We intended to establish prerequisites for such studies in animal models and to show the feasibility of this approach in rabbits. Isolated articular chondrocytes were retrovirally marked using green fluor...

journal_title：Osteoarthritis and cartilage

authors： Hirschmann F,Verhoeyen E,Wirth D,Bauwens S,Hauser H,Rudert M

更新日期：2002-02-01 00:00:00

abstract：OBJECTIVE:This study aims to apply gene expression profiling technology to gain insight into the molecular regulation of mesenchymal chondrogenesis. METHODS:The experimental system consists of micromass cultures of C3H10T1/2 cells, a murine multipotential embryonic cell line, treated with the chondroinductive growth f...

journal_title：Osteoarthritis and cartilage

authors： Izzo MW,Pucci B,Tuan RS,Hall DJ

更新日期：2002-01-01 00:00:00

abstract：OBJECTIVE:The effect of mechanical injury on chondrocyte viability and matrix degradation was studied. It was proposed that mechanical injury to human cartilage explants results in chondrocyte apoptosis with associated loss of glycosaminoglycans. DESIGN:Full thickness human cartilage explants, 5 mm in diameter were su...

journal_title：Osteoarthritis and cartilage

authors： D'Lima DD,Hashimoto S,Chen PC,Colwell CW Jr,Lotz MK

更新日期：2001-11-01 00:00:00

abstract：OBJECTIVES:Determine the depth-varying confined and osmotic compression moduli of normal human articular cartilage from the femoral head, and test whether these moduli are dependent on fixed charge density. METHODS AND RESULTS:Using an automated instrument to allow epifluorescence microscopy analysis during confined c...

journal_title：Osteoarthritis and cartilage

authors： Chen SS,Falcovitz YH,Schneiderman R,Maroudas A,Sah RL

更新日期：2001-08-01 00:00:00

abstract：OBJECTIVES:To examine the in situ distributions of vitamin D receptors (VDR) and matrix metalloproteinases (MMPs) in osteoarthritic cartilage for comparison with non-arthritic, normal cartilage; and to assess the in vitro effects of 1alpha,25 dihydroxyvitaminD(3)(1alpha,25(OH)(2)D(3)) on MMPs-1, -3 and -9 and prostagla...

journal_title：Osteoarthritis and cartilage

authors： Tetlow LC,Woolley DE

更新日期：2001-07-01 00:00:00

abstract：OBJECTIVE:Adult articular cartilage is a physiologically hypoxic tissue with a proposed gradient of oxygen tension ranging from <10% oxygen at the cartilage surface to <1% in the deepest layers. This gradient may be disturbed during diseases of the joint, for example in rheumatoid arthritis when synovial fluid pO(2)fal...

journal_title：Osteoarthritis and cartilage

authors： Grimshaw MJ,Mason RM

更新日期：2001-05-01 00:00:00

abstract：OBJECTIVE:Reliable evaluation of joint space width and subchondral sclerosis of osteoarthritic joints is difficult. The present study describes a new digital method to analyse standard radiographs of the ankle. DESIGN:Standardized radiographs were taken of the ankle of 12 patients with severe osteoarthritis (OA) under...

journal_title：Osteoarthritis and cartilage

authors： Marijnissen AC,Vincken KL,Viergever MA,van Roy HL,Van Roermund PM,Lafeber FP,Bijlsma JW

更新日期：2001-04-01 00:00:00

abstract：OBJECTIVE:To investigate the impact of a prolonged and constant active TGF-beta expression by the synovial lining cells on cartilage and ligamentous joint structures in vivo. DESIGN:An adenoviral vector (AdTGF-beta1(223,225)) was used for the overexpression of active TGF-beta1 in knee joints of C57Bl/6 mice. RESULTS:...

journal_title：Osteoarthritis and cartilage

authors： Bakker AC,van de Loo FA,van Beuningen HM,Sime P,van Lent PL,van der Kraan PM,Richards CD,van den Berg WB

更新日期：2001-02-01 00:00:00

abstract：OBJECTIVE:Quantitation and analyses of the fine structure of glycosaminoglycans are increasingly important for understanding many biological processes, including those most critical for understanding skeletal biology. We have developed a novel procedure, fluorophore-assisted carbohydrate electrophoresis (FACE), for det...

journal_title：Osteoarthritis and cartilage

authors： Calabro A,Midura R,Wang A,West L,Plaas A,Hascall VC

更新日期：2001-01-01 00:00:00

abstract：OBJECTIVE:Because articular cartilage has limited ability to repair itself, treatment of (osteo)chondral lesions remains a clinical challenge. We aimed to evaluate how well a tissue-engineered cartilage-like implant, derived from chondrocytes cultured in a novel patented, scaffold-free bioreactor system, would perform ...

journal_title：Osteoarthritis and cartilage

authors： Mainil-Varlet P,Rieser F,Grogan S,Mueller W,Saager C,Jakob RP

更新日期：2001-01-01 00:00:00

abstract：OBJECTIVE:To develop flow cytometry for the study of human articular cartilage cell phenotype and to validate the method on chondrocytes cultured in different in-vitro systems. METHODS:Chondrocyte phenotype was modulated by culturing the cells under different in-vitro conditions: i.e. in monolayer and in suspension cu...

journal_title：Osteoarthritis and cartilage

authors： Wang L,Verbruggen G,Almqvist KF,Elewaut D,Broddelez C,Veys EM

更新日期：2001-01-01 00:00:00

abstract：OBJECTIVE:To characterize a novel secreted frizzled-related protein (SFRP) and determine its tissue distribution at the mRNA and protein level. METHODS:The FrzB-2 gene was identified by expressed sequence tag (EST) analysis of human tissue-derived libraries. Tissue distribution of FrzB-2 mRNA was determined by Norther...

journal_title：Osteoarthritis and cartilage

authors： James IE,Kumar S,Barnes MR,Gress CJ,Hand AT,Dodds RA,Connor JR,Bradley BR,Campbell DA,Grabill SE,Williams K,Blake SM,Gowen M,Lark MW

更新日期：2000-11-01 00:00:00

abstract：OBJECTIVES:The objective of this study was to evaluate the oral combination of glucosamine HCl, sodium chondroitin sulfate and manganese ascorbate for the treatment of osteoarthritis (OA) of the knee. DESIGN:A randomized placebo-controlled study design was implemented. We recruited 93 patients with OA of the knee from...

journal_title：Osteoarthritis and cartilage

authors： Das A Jr,Hammad TA

更新日期：2000-09-01 00:00:00

abstract：:Objective To determine whether meniscal cells can express a TGFbeta(1)transgene delivered by a retroviral vector, and respond to the gene product by increasing matrix synthesis. Methods Monolayer cultures of human and canine meniscal cells were infected with retroviruses carrying either a human TGFbeta(1)cDNA or marke...

journal_title：Osteoarthritis and cartilage

authors： Goto H,Shuler FD,Niyibizi C,Fu FH,Robbins PD,Evans CH

更新日期：2000-07-01 00:00:00

abstract：OBJECTIVE:Marathon runners have an increased risk of developing joint disease. During and after a 42-km run, elevation of multiple cytokines occurs in the blood, reflecting inflammatory processes. We compared this cytokine response with serum levels of cartilage oligomeric matrix protein (COMP) and melanoma inhibitory ...

journal_title：Osteoarthritis and cartilage

authors： Neidhart M,Müller-Ladner U,Frey W,Bosserhoff AK,Colombani PC,Frey-Rindova P,Hummel KM,Gay RE,Häuselmann H,Gay S

更新日期：2000-05-01 00:00:00

abstract：OBJECTIVE:To employ a magnetic resonance (MR) imaging technique for quantitative assessment of thin cartilage layers, and to validate the cartilage volume and thickness measurements. METHODS:We investigated 10 normal elbow joints (age 20 to 69 years) with a 3D gradient echo sequence with selective water excitation (TR...

journal_title：Osteoarthritis and cartilage

authors： Graichen H,Springer V,Flaman T,Stammberger T,Glaser C,Englmeier KH,Reiser M,Eckstein F

更新日期：2000-03-01 00:00:00